Cobalt chloride enhances angiogenic potential of CD 133 + cells
نویسندگان
چکیده
1. Abstract 2. Introduction 3. Materials and methods 3.1. Human CD133 cell isolation 3.2. Cell culture 3.3. Acetylated LDL uptake and UEA-1 lectin binding site labeling 3.4. Tubule formation 3.5. Flow cytometric analysis of cell surface markers 3.6. Migration assay 3.7. Cell wound healing 3.8. Cell proliferation Assay 3.9. Measurement of VEGF, FGF, and HIF-1α protein by ELISA 3.10. Real time PCR 3.11. Western blot analysis 3.12. Statistical analysis 4. Results 4.1. Characteristics of isolated CD133 positive cells from umbilical cord blood 4.2. Cell migration 4.3. Proliferation 4.4. Tubule formation ability 4.5. Secretion of growth factors by CD133 4.6. Hypoxia induced the protein expression 4.7. HIF-1α and downstream gene expression 5. Discussion 6. Conclusion 7. Acknowledgements 8. References
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